Molecular Methods - Revision history

Bradley Monk at 05:20, 10 August 2014

2014-08-10T05:20:55Z

← Older revision		Revision as of 22:20, 9 August 2014
Line 46:		Line 46:


	[[Category:Neuroscience Methods]] [[Category:ReDiClus]]		[[Category:Neuroscience Methods]] [[Category:ReDiClus]][[Category:Neurobiology]]

2013-08-05T02:36:12Z

← Older revision		Revision as of 19:36, 4 August 2013
Line 46:		Line 46:


	[[Category:Neuroscience Methods]]		[[Category:Neuroscience Methods]] [[Category:ReDiClus]]

2013-07-05T01:34:48Z

← Older revision		Revision as of 18:34, 4 July 2013
Line 1:		Line 1:
			{{PageHead\|[[Malinow]]\|[[Molecular Methods]]\|[[Quantum Dots]]\|[[Choquet]]\|[[AMPAR]]}}

			==Zac Email==
			Hi Guys,
			Here are the different labeling techniques that might be applicable with recombinant expression of AMPARs. Roughly ranked from most to least likely to succeed, separated by large vs small [[AMPAR]] N-terminal additions. The references in parentheses are for background on the technique.


			:'''Large [[AMPAR]] N-terminal addition'''
			:* Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate
			:* [[AMPAR]]-streptavidin, QD-biotin

			:'''Small [[AMPAR]] N-terminal addition'''
			:* [[AMPAR]]-FLAG, QD-anti-FLAG
			:* [[AMPAR]]-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)
			:* [[AMPAR]]-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)
			:* [[AMPAR]]-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)


	==Fluorescence Imaging==		==Fluorescence Imaging==

2013-07-03T23:05:39Z

← Older revision		Revision as of 16:05, 3 July 2013
Line 10:		Line 10:
	:* [http://en.wikipedia.org/wiki/Super_resolution_microscopy#Photoactivated_localization_microscopy_.28PALM.29_and_FPALM PALM STORM]		:* [http://en.wikipedia.org/wiki/Super_resolution_microscopy#Photoactivated_localization_microscopy_.28PALM.29_and_FPALM PALM STORM]
	:* [http://en.wikipedia.org/wiki/Fluorescence-lifetime_imaging_microscopy Fluorescence-lifetime imaging microscopy or FLIM]		:* [http://en.wikipedia.org/wiki/Fluorescence-lifetime_imaging_microscopy Fluorescence-lifetime imaging microscopy or FLIM]
			:* [http://en.wikipedia.org/wiki/Two-photon_excitation_microscopy Two-photon Microscopy]

2013-07-03T23:04:12Z

← Older revision		Revision as of 16:04, 3 July 2013
Line 9:		Line 9:
	; Microscopy		; Microscopy
	:* [http://en.wikipedia.org/wiki/Super_resolution_microscopy#Photoactivated_localization_microscopy_.28PALM.29_and_FPALM PALM STORM]		:* [http://en.wikipedia.org/wiki/Super_resolution_microscopy#Photoactivated_localization_microscopy_.28PALM.29_and_FPALM PALM STORM]
			:* [http://en.wikipedia.org/wiki/Fluorescence-lifetime_imaging_microscopy Fluorescence-lifetime imaging microscopy or FLIM]


			; See Also
			:* [http://www.ncbi.nlm.nih.gov/pubmed/20434995 Fluorescence applications in molecular neurobiology]

2013-07-03T22:01:52Z

← Older revision		Revision as of 15:01, 3 July 2013
Line 1:		Line 1:

	==Fluorescence Imaging==		==Fluorescence Imaging==
			<br>
	; Molecular Interaction or Proximity		; Molecular Interaction or Proximity
	:* [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET		:* [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET
Line 6:		Line 7:


			; Microscopy
			:* [http://en.wikipedia.org/wiki/Super_resolution_microscopy#Photoactivated_localization_microscopy_.28PALM.29_and_FPALM PALM STORM]

2013-07-03T21:58:19Z

← Older revision		Revision as of 14:58, 3 July 2013
Line 1:		Line 1:

	==Fluorescence Imaging==		==Fluorescence Imaging==
			; Molecular Interaction or Proximity
			:* [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET
			:* [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]

2013-07-03T21:52:43Z

Created page with " ==Fluorescence Imaging== Category:Neuroscience Methods"

New page

==Fluorescence Imaging==

[[Category:Neuroscience Methods]]