Cloning Protocol - Revision history

Bradley Monk at 22:37, 13 January 2016

2016-01-13T22:37:25Z

Bradley Monk at 22:58, 11 January 2016

2016-01-11T22:58:03Z

@@ Line 1: / Line 1: @@
 === Step 1: Phusion PCR ===
 Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL)
 <!-- <mediaplayer width='500' height='300'>http://www.bradleymonk.com/w/images/c/cc/Cloning1.mp4</mediaplayer> -->
 {{#widget:Html5media|url=http://www.bradleymonk.com/w/images/c/cc/Cloning1.mp4|width=400}}
 {|
 |-
-| 10 uM forward primer || &nbsp; &nbsp; || 10 uL
+| xxxxx ||  xxxxx
 |-
-| 10 uM reverse primer || || 10 uL
+| xxxxx ||| xxxxx
 |-
-| 2x phusion master mix (MM) || || 100 uL
+| xxxxx || xxxxx
 |-
-| 10 ng total template || || 1 uL total
+| xxxxx || xxxxx
 |-
-| H2O up to 200 uL || || variable - top to 200 uL
+| xxxxx || xxxxx
 |}

Bradley Monk at 18:31, 11 January 2016

2016-01-11T18:31:05Z

← Older revision		Revision as of 11:31, 11 January 2016
Line 1:		Line 1:


	Step 1: Phusion PCR
			=== Step 1: Phusion PCR ===

	Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL)		Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL)

			<!-- <mediaplayer width='500' height='300'>http://www.bradleymonk.com/w/images/c/cc/Cloning1.mp4</mediaplayer> -->
			{{#widget:Html5media\|url=http://www.bradleymonk.com/w/images/c/cc/Cloning1.mp4\|width=400}}

	{\|		{\|

Bradley Monk at 18:02, 11 January 2016

2016-01-11T18:02:53Z

← Older revision		Revision as of 11:02, 11 January 2016
Line 8:		Line 8:
	{\|		{\|
	\|-		\|-
	\| 10 uM forward primer \|\| 10 uL		\| 10 uM forward primer \|\|     \|\| 10 uL
	\|-		\|-
	\| 10 uM reverse primer \|\| 10 uL		\| 10 uM reverse primer \|\| \|\| 10 uL
	\|-		\|-
	\| 2x phusion master mix (MM) \|\| 100 uL		\| 2x phusion master mix (MM) \|\| \|\| 100 uL
	\|-		\|-
	\| 10 ng total template \|\| 1 uL total		\| 10 ng total template \|\| \|\| 1 uL total
	\|-		\|-
	\| H2O up to 200 uL \|\| variable - top to 200 uL		\| H2O up to 200 uL \|\| \|\| variable - top to 200 uL
	\|}		\|}

Bradley Monk: Created page with " Step 1: Phusion PCR Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL) {| |- | 10 uM forward primer || 10 uL |- | 10 uM reverse primer || 10 uL |- | 2x phusion..."

2016-01-11T18:00:20Z

Created page with " Step 1: Phusion PCR Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL) {| |- | 10 uM forward primer || 10 uL |- | 10 uM reverse primer || 10 uL |- | 2x phusion..."

New page

Step 1: Phusion PCR

Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL)

{|
|-
| 10 uM forward primer || 10 uL
|-
| 10 uM reverse primer || 10 uL
|-
| 2x phusion master mix (MM) || 100 uL
|-
| 10 ng total template || 1 uL total
|-
| H2O up to 200 uL || variable - top to 200 uL
|}