Choquet - Revision history

Bradley Monk at 05:21, 10 August 2014

2014-08-10T05:21:56Z

← Older revision		Revision as of 22:21, 9 August 2014
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	</pre>		</pre>

	[[Category:Malinow]] [[Category:ReDiClus]]		[[Category:Malinow]] [[Category:ReDiClus]][[Category:Neurobiology]]
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Bradley Monk at 02:36, 5 August 2013

2013-08-05T02:36:31Z

← Older revision		Revision as of 19:36, 4 August 2013
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	[[Category:Malinow]]		[[Category:Malinow]] [[Category:ReDiClus]]
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Bradley Monk at 02:22, 15 July 2013

2013-07-15T02:22:54Z

← Older revision		Revision as of 19:22, 14 July 2013
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	{{PageHead\|[[Malinow]]\|[[Molecular Methods]]\|[[Quantum Dots]]\|[[Choquet]]\|[[AMPAR]]}}		{{PageHead\|[[Malinow]]\|[[Molecular Methods]]\|[[Quantum Dots]]\|[[Choquet]]\|[[AMPAR]]}}
			[[Category:Malinow]]

	<div style="font-size:24px; font-family:Century Gothic;">Study Timeline [http://www.ncbi.nlm.nih.gov/pubmed?term=Choquet%20D%5BAuthor%5D&cauthor=true&cauthor_uid=12970178 - PubMed]</div>		<div style="font-size:24px; font-family:Century Gothic;">Study Timeline [http://www.ncbi.nlm.nih.gov/pubmed?term=Choquet%20D%5BAuthor%5D&cauthor=true&cauthor_uid=12970178 - PubMed]</div>
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	{{Article\|Author\|Year\|Journal - [http://bradleymonk.com/media/Choquet1.pdf PDF]\|15749166\|Title}}		{{Article\|Author\|Year\|Journal - [http://bradleymonk.com/media/Choquet1.pdf PDF]\|15749166\|Title}}
	</pre>		</pre>

			[[Category:Malinow]]
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Bradley Monk at 09:53, 14 July 2013

2013-07-14T09:53:46Z

← Older revision		Revision as of 02:53, 14 July 2013
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	; GluR1 QDots diffusion rate		; {{1\|GluR1}} QDots diffusion rate
	* high - extrasynaptic		* high - extrasynaptic
	* medium - inactivated synapses		* medium - inactivated synapses
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	;GluR1 QDots features		;{{1\|GluR1}} QDots features
	* often passed through silenced synapses		* often passed through silenced synapses
	* often moved from silenced to active synapse		* often moved from silenced to active synapse
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	;GluR1 QDots diffusion rate after synaptic reversal		;{{1\|GluR1}} QDots diffusion rate after synaptic reversal
	* {{Nc}} at previously active synapses		* {{Nc}} at previously active synapses
	* {{Nc}} at previously silenced synapses		* {{Nc}} at previously silenced synapses

Bradley Monk at 09:51, 14 July 2013

2013-07-14T09:51:46Z

← Older revision		Revision as of 02:51, 14 July 2013
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	{{Article\|Ehlers, Heine, Groc, Lee, Choquet\|2007\|Neuron - [http://bradleymonk.com/media/Choquet2007B.pdf PDF]\|17481397\|Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity}}		{{Article\|Ehlers, Heine, Groc, Lee, Choquet\|2007\|Neuron - [http://bradleymonk.com/media/Choquet2007B.pdf PDF]\|17481397\|Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity}}

	;~~Silenced~~ synapses (compared to active synapses)		;Expression at silenced synapses compared to active synapses
	* {{Dn}} (50%) GluR1		* {{Dn}} (50%) GluR1
	* {{Nc}} PSD-95		* {{Nc}} PSD-95
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	;GluR1 QDots diffusion rate		; GluR1 QDots diffusion rate
	* high - extrasynaptic		* high - extrasynaptic
	* medium - inactivated synapses		* medium - inactivated synapses

Bradley Monk: /* Experiment Ideas */

2013-07-13T06:16:10Z

Experiment Ideas

← Older revision		Revision as of 23:16, 12 July 2013
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	----		----
	<big>Zac Email</big>		<big>Zac Email</big><br>
	Here are the different labeling techniques that might be applicable with recombinant expression of AMPARs. Roughly ranked from most to least likely to succeed, separated by large vs small AMPAR N-terminal additions. The references in parentheses are for background on the technique.		Here are the different labeling techniques that might be applicable with recombinant expression of AMPARs. Roughly ranked from most to least likely to succeed, separated by large vs small AMPAR N-terminal additions. The references in parentheses are for background on the technique.

Bradley Monk: /* Experiment Ideas */

2013-07-13T06:15:00Z

Experiment Ideas

← Older revision	Revision as of 23:15, 12 July 2013
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		----
		<big>Zac Email</big>
		Here are the different labeling techniques that might be applicable with recombinant expression of AMPARs. Roughly ranked from most to least likely to succeed, separated by large vs small AMPAR N-terminal additions. The references in parentheses are for background on the technique.

		:'''Large AMPAR N-terminal addition'''
		:* Halotag (Promega): AMPAR-enzyme, QD-Halotag substrate
		:* AMPAR-streptavidin, QD-biotin

		:'''Small AMPAR N-terminal addition'''
		:* AMPAR-FLAG, QD-anti-FLAG
		:* AMPAR-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)
		:* AMPAR-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)
		:* AMPAR-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)

Bradley Monk: /* Experiment Ideas */

2013-07-13T06:12:20Z

Experiment Ideas

← Older revision		Revision as of 23:12, 12 July 2013
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	<big>Choquet 2010</big>

			----
			<big>Choquet 2010</big> '''CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of stargazin'''
	* NMDAR activation promotes rapid translocation of aCaMKII::GFP to synapses, causing AMPAR trapping at 1 min (only synapses with CaMKII translocation)		* NMDAR activation promotes rapid translocation of aCaMKII::GFP to synapses, causing AMPAR trapping at 1 min (only synapses with CaMKII translocation)
	* tCaMKII (active prion) promotes immobilization of endogenous GluR1 (containing) AMPARs (both synaptic and extrasynaptic), and to a much lesser extent GluA2 (containing) AMPARs.		* tCaMKII (active prion) promotes immobilization of endogenous GluR1 (containing) AMPARs (both synaptic and extrasynaptic), and to a much lesser extent GluA2 (containing) AMPARs.
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	* findings consistent with specific role of GluA1 in activity-dependent trafficking - but Stargazin can bind all subunits??		* findings consistent with specific role of GluA1 in activity-dependent trafficking - but Stargazin can bind all subunits??
	* findings raise possibility that during LTP, CaMKII activation triggers both classical LTP and PPD. Interesting that LTP is frequently accompanied by PPD (opposite of PPF: paired-pulse facilitation)		* findings raise possibility that during LTP, CaMKII activation triggers both classical LTP and PPD. Interesting that LTP is frequently accompanied by PPD (opposite of PPF: paired-pulse facilitation)
			----

Bradley Monk: /* Experiment Ideas */

2013-07-13T06:09:48Z

Experiment Ideas

← Older revision		Revision as of 23:09, 12 July 2013
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	==Experiment Ideas==		==Experiment Ideas==
	{{ExpandBox\|~~Expand To View~~\|		{{ExpandBox\|experimental notes and highlighted findings\|
	~~Ideas Here~~

			<big>Choquet 2010</big>
			* NMDAR activation promotes rapid translocation of aCaMKII::GFP to synapses, causing AMPAR trapping at 1 min (only synapses with CaMKII translocation)
			* tCaMKII (active prion) promotes immobilization of endogenous GluR1 (containing) AMPARs (both synaptic and extrasynaptic), and to a much lesser extent GluA2 (containing) AMPARs.
			* CaMKII direct phosphorylation of AMPARs unnecessary for synaptic trapping
			* GluA1 - SAP97 interaction unnecessary for CaMKII-dependent synaptic trapping
			* Stargazin increased tCaMKII-mediated trapping of recombinant GluA1 (homomeric), but tCaMKII had no effect on mobility of recombinant GluA2 (homomeric)
			* Stargazin phosphorylation (by tCaMKII) is necessary for GluA1 trapping; blocking phosphorylation caused AMPAR mobility to significantly increase.
			* intriguing finding: GluA1 subunit-specific effect of CaMKII, where it immobilizes recombinant GluA1 but not GluA2 homomeric AMPARs.
			* findings consistent with specific role of GluA1 in activity-dependent trafficking - but Stargazin can bind all subunits??
			* findings raise possibility that during LTP, CaMKII activation triggers both classical LTP and PPD. Interesting that LTP is frequently accompanied by PPD (opposite of PPF: paired-pulse facilitation)


	}}<!-- END ARTICLE -->		}}<!-- END ARTICLE -->

Bradley Monk: /* Most Relevant Studies */

2013-07-13T06:08:08Z

Most Relevant Studies

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 {{ExpandBox|2010 CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of stargazin|
 {{Article|Opazo P, Labrecque S, Tigaret CM, Frouin A, Wiseman PW, De Koninck P, Choquet D.|2010|Neuron - [http://bradleymonk.com/media/Choquet2010A.pdf PDF]|20670832|CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of stargazin}}
 <big>Abstract</big>