https://bradleymonk.com/wiki/api.php?action=feedcontributions&feedformat=atom&user=Karishma+Pratt 2026-04-09T16:31:10Z User contributions MediaWiki 1.41.0 https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=2074 2013-10-01T05:37:47Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * [[#superecliptic phluorin SEP]]<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> * [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339873/pdf/jove-50-2568.pdf FRAP]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> * [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690942/ sptPALM]<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Box|Cell Culture|width=45%|<br /> * [http://www.lifetechnologies.com/us/en/home/references/protocols/neurobiology/neurobiology-protocols/isolation-culture-and-characterization-of-cortical-and-hippocampal-neurons.html Rat Hippocampus Neurons E18]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=2048 2013-09-13T21:49:10Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * [[#superecliptic phluorin SEP]]<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> * [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339873/pdf/jove-50-2568.pdf FRAP]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Box|Cell Culture|width=45%|<br /> * [http://www.lifetechnologies.com/us/en/home/references/protocols/neurobiology/neurobiology-protocols/isolation-culture-and-characterization-of-cortical-and-hippocampal-neurons.html Rat Hippocampus Neurons E18]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=2047 2013-09-13T21:46:18Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * [[#superecliptic phluorin SEP]]<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> * [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339873/pdf/jove-50-2568.pdf FRAP]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Box|Cell Culture|width=45%|<br /> * Rat Hippocampus Neurons E18<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=2046 2013-09-13T21:44:24Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * [[#superecliptic phluorin SEP]]<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> * [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339873/pdf/jove-50-2568.pdf FRAP]]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Box|Cell Culture|width=45%|<br /> * Rat Hippocampus Neurons E18<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=2012 2013-09-07T01:50:52Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * [[#superecliptic phluorin SEP]]<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Box|Cell Culture|width=45%|<br /> * Rat Hippocampus Neurons E18<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=2011 2013-09-07T01:50:04Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * [[#superecliptic phluorin SEP]]<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Box|Cell Culture|width=45%|<br /> * Rat Hippocampus Neurons E18<br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=Sandbox&diff=1958 2013-08-27T21:49:57Z

<p>Karishma Pratt: </p> <hr /> <div>crash course time<br /> <br /> yo wus good<br /> <br /> [[Sandbox2|yo wus good]]<br /> <br /> {{:Sandbox2}}</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=Sandbox&diff=1953 2013-08-27T21:33:59Z

<p>Karishma Pratt: </p> <hr /> <div>crash course time<br /> <br /> yo wus good<br /> <br /> [[Sandbox2|yo wus good]]</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=Sandbox&diff=1950 2013-08-27T21:30:04Z

<p>Karishma Pratt: </p> <hr /> <div>crash course time<br /> <br /> yo wus good</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=1945 2013-08-27T20:23:46Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * [[#superecliptic phluorin SEP]]<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=1944 2013-08-27T20:20:24Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * [[#superecliptic_phluorin_SEP|superecliptic phluorin (SEP)]]<br /> * [[#superecliptic phluorin SEP]]<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=1943 2013-08-27T20:16:57Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * [[User_talk:Karishma_Pratt#superecliptic_phluorin_SEP|superecliptic phluorin (SEP)]]<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=1942 2013-08-27T20:15:47Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * superecliptic phluorin (SEP)<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin SEP==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=1941 2013-08-27T20:14:15Z

<p>Karishma Pratt: </p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * superecliptic phluorin (SEP)<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * streptavidin, QD-biotin<br /> * FLAG, QD-anti-FLAG<br /> * biotin ligase recognition peptide<br /> * unnatural amino acid azide, QD-propargyl<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> <br /> ==superecliptic phluorin (SEP)==<br /> superecliptic phluorin (SEP) are used to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=1940 2013-08-27T20:11:26Z

<p>Karishma Pratt: /* FRAP */ new section</p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * superecliptic phluorin (SEP)<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * AMPAR-streptavidin, QD-biotin<br /> * AMPAR-FLAG, QD-anti-FLAG<br /> * AMPAR-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)<br /> * AMPAR-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)<br /> * AMPAR-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Clear}}<br /> <br /> == FRAP ==<br /> <br /> FRAP is a technique to do this...</div>

Karishma Pratt https://bradleymonk.com/wiki/index.php?title=User_talk:Karishma_Pratt&diff=1939 2013-08-27T20:09:09Z

<p>Karishma Pratt: Created page with &quot;{{Box|Receptor Protein Tags|width=45%| * fluorescent proteins * superecliptic phluorin (SEP) * photoactivatable green fluorescent protein (PAGFP) }} {{Box|Protein Quantificat...&quot;</p> <hr /> <div>{{Box|Receptor Protein Tags|width=45%|<br /> * fluorescent proteins<br /> * superecliptic phluorin (SEP)<br /> * photoactivatable green fluorescent protein (PAGFP)<br /> }}<br /> <br /> {{Box|Protein Quantification|width=45%|<br /> * Western Blot<br /> * Quantitative Autoradiography<br /> }}<br /> <br /> {{Clear}}<br /> <br /> {{Box|Molecular Tracking|width=45%|<br /> * Quantum Dot Tagging<br /> * Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate<br /> * AMPAR-streptavidin, QD-biotin<br /> * AMPAR-FLAG, QD-anti-FLAG<br /> * AMPAR-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)<br /> * AMPAR-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)<br /> * AMPAR-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)<br /> }}<br /> <br /> {{Box|Protein Interaction|width=45%|<br /> * FRET<br /> * Two Hybrid Screening<br /> * [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET<br /> * [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]<br /> }}<br /> <br /> {{Clear}}</div>

Karishma Pratt